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Changhe Wang

Professor, Ph.D.

Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology

Xi’an Jiaotong University,

No. 28, Xianning West Road, Xi’an, 710049, China

Tel: +86-17791408270;

Email: changhewang@xjtu.edu.cn; changhecool@163.com

 

RESEARCH INTEREST

With our expertise on neurobiology, especially electrophysiological recordings (patch clamp, UV-flash, sniffer patch, Ca2+ imaging), electrochemical recordings (amperometric current at quantal, cellular and in  vivo level), bio-imaging (confocal, STED, TIRF, two photon, EM), opto-genetic/chemical-genetic stimulation, in vivo virus infection and molecular/biochemical assays, our ongoing research is aimed to address some of the most important questions in the molecular mechanism underlying exocytosis, endocytosis and vesicle recycling during synaptic transmission and in the diseased brain:

1)  Mechanisms of vesicle docking, priming and fusion processes, especially the fusion machinery, fusion modes & fusion-pore dilation.

2)  Mechanisms of exo-endocytosis coupling, vesicle recycling and the sustained neurotransmission.

3)  Synaptic transmission and synaptic plasticity in health and diseased brain, e.g. Parkinson’s diseases (PD), Alzheimer’s disease (AD) and other neural disorders.

 

EDUCATION

2008-2012  Ph.D. in Physiology (Thesis advisors: Drs. Zhuan Zhou & Claire Xi Zhang), Peking University (PKU), China.

2003-2006  M.S. in Zoology (Thesis advisor: Dr. Cong Guo), Sichuan University (SCU), China.

2000-2003  B.S. in Bioscience (SCU-University of Washington Joint program), SCU, China.

 

POSITIONS

2016-present Professor, School of Life Science and Technology, Xi’an Jiaotong University, China

2012-2015 Postdoctoral Fellow (with Dr. Zhuan Zhou), Peking University, China

2006-2008 Teaching assistant & Lecturer, Qiqihar University, China

 

FELLOWSHIPS AND AWARDS

2019 Winner of Shitsan Pai Award for Young Biophysicists

2018 Winner of Natural Science Fund for Outstanding Scholars of Shaanxi Province, China.

2017 The first prize of Science Research Famous Achievement Award in Higher Institution (Natural Science Award, the 3rd contributor), Ministry of Education, China.

2017 Hundred Talents Program of Shaanxi Province, Shaanxi Province Government, China.

2016 Excellent Postdoctoral Fellowship of Peking University, Peking University, China.

2015 Young Talent Support Plan of Xi’an Jiaotong University, Xi’an Jiaotong University, China.

2014 Excellent Postdoctoral Fellowship of Peking-Tsinghua Center for Life Sciences, Peking University, China.

2014 The second prize for academic reports of State key laboratory of biomembrane and membrane biotechnology, Peking University, China.

2008-2012 Academic scholarship, in Dr. Zhuan Zhou lab, Institute of Molecular Medicine, Peking University, China.

2009 Robin Li scholarship, Dr. Zhuan Zhou lab, Institute of Molecular Medicine, Peking University, China.

2004 The first prize for Guanghua scholarship, Dr. Cong Guo lab, College of Life Sciences, Sichuan University, China.

2003 The second prize graduate scholarship, Dr. Cong Guo lab, College of Life Sciences, Sichuan University, China.

2002 The third prize of excellent undergraduate thesis, Dr. Cong Guo lab, Wu Yuzhang Honors College, Sichuan University, China.

2001 Undergraduate grade-skipping, Wu Yuzhang Honors College, Sichuan University, China.

2001 ‘Outstanding youth of SCU-UW (University of Washington) program’ honor, Wu Yuzhang Honors College, Sichuan University, China.

2000 Taichanzu temple scholarship, , Wu Yuzhang Honors College, Sichuan University, China.

 

ORGANISATION OF SCIENTIFIC MEETINGS

2018 Organizing Committee number of the 12th international Symposium on Calcium Signaling in China, Shenyang, China

2018 Symposium chair for ‘Neurobiophysics – Cell & Molecule’ in the 16th Chinese Biophysics Congress, Chengdu, China

2018 Symposium chair for ‘Joint Session – Israeli Society of Physiology and Parmacology and Austrian Biophysical Society’ in the 16th Chinese Biophysics Congress, Chengdu, China

2016 Organizing Committee number of the 11th international Symposium on Calcium Signaling in China, Zunyi, China

2014 Symposium chair for ‘sympathetic nerve activity and cardiogenic sudden death’ in the 24th Congress of Chinese Association for Physiological Sciences, Shanghai, China

 

MEMBERSHIPS OF SCIENTIFIC SOCIETIES

2016-2020 Member and secretory of the Neural BioPhysical Committee, Biophysical Society of China

2017-2020 Member of the Scientific Committee of Aging and Antiaging, Gerontological Society of China

2017-2021 Academic committee member of CAMPUS Asia Project

2018-2021 Popular science expertise for China Science and Technology Press

2018-2022 Council member of the Cell Biology Society of Shaan Xi Province

2012-2016 Council member of the Proteome Society of Heilongjiang Province

2011-2015 Council member of the Microbiology Society of Heilongjiang Province

 

Selected Peer-Reviewed Publications

1.      Rong Huang, Yuan Wang, Jie Li, Xiaohan Jiang, Yinglin Li, Bing, Liu, Xi Wu, Xingyu Du, Yuqi Hang, Mu Jin, Feipeng Zhu, Changhe Wang#, Zuying Chai#, Zhuan Zhou#. (2019) Ca2+-independent but voltage-dependent quantal catecholamine secretion (CiVDS) in the mammalian synpathetic nervous system. Proc Natl Acad Sci U S A, doi/10.1073/pnas.1902444116. (Epub ahead of print, IF: 9.5, #Corresponding authors).

2.      Xuanang Wu, Shaoqin Hu, Xinjiang Kang#, and Changhe Wang#. (2019) Synaptotagmins: beyond presynaptic neurotransmitter release. Neuroscientist. DOI: 10.1177/1073858419844497. (Epub ahead of print, IF: 7.46, # Corresponding authors) .

3.      Mingzhu Huang, Bianbian Wang, Xiaopeng Li, Chongluo Fu#, Changhe Wang#, and Xinjiang Kang#. (2019) ɑ-Synuclein: a multifunctional player in exocytosis, endocytosis, and vesicle recycling. Frontiers in Neuroscience. 13:28. doi: 10.3389/fnins.2019.00028. (IF: 3.88, # Corresponding authors) (Invited review)

4.      Qian Song, Mingzhu Huang, Bianbian Wang, Xinjiang Kang, Wang Changhe#. (2018) Bidirectional regulation of Ca2+ in exo-endocytosis coupling. Science China Life Sciences. 61(12):1583-1585. (IF: 3.09, # Corresponding author) (Invited review)

5.      Changhe Wang *, Xinjiang Kang *, Li Zhou *, Zuying Chai, Qihui Wu, Rong Huang, Huadong Xu, Meiqin Hu, Xiaoxuan Sun, Suhua Sun, Jie Li, Ruiying Jiao, Panli Zuo, Lianghong Zheng, Zhenyu Yue, and Zhuan Zhou. (2018) Synaptotagmin-11 is a critical mediator of parkin-linked neurotoxicity and Parkinson’s Disease like pathology. Nature Communications. 9:81. doi:10.1038/s41467-017-02593-y. (IF: 12.35, *Co-first authors).

Highlighted in Movement Disorders (Moghaddam HS and Arabi MH, Synaptotagmin-11 is a novel hotspot in the pathogenesis of parkin-linked Parkinson’s disease: new implications for clinical targeting, 2018, 33(4):582).

6.      Zuying Chai *, Changhe Wang *, Rong Huang*, Yuan Wang*, Xiaoyu Zhang, Qihui Wu, Yeshi Wang, Xi Wu, Lianghong Zheng, Chen Zhang, Wei Guo, Wei Xiong, Jiuping Ding, Feipeng Zhu, and Zhuan Zhou. (2017) CaV2.2 gates calcium-independent but voltage-dependent secretion in mammalian sensory neurons. Neuron. 96(6):1317-26. (IF: 14.32, *Co-first authors).

Highlighted in Nature Reviews Neuroscience (Natasha Bray, A discrete form of secretion, 2017, 19(1):6. doi: 10.1038/nrn.2017.162).

Recommended by the Faculty of 1000 (F1000Prime recommendations by Ronald W Holz, F1000Prime recommendations by Yang Yang, and F1000Prime recommendations by William Colmers).

7.      Yeshi Wang, Qihui Wu, Meiqin Hu, Bin Liu, Zuying Chai, Rong Huang, Yuan Wang, Zhou Li, Xu Huadong, Changhe Wang#, and Zhuan Zhou#. (2017) Ligand- and voltage-gated Ca2+ channels differentially regulate the mode of vesicular neuropeptide release in mammalian sensory neurons. Science Signaling. 10(484), eaal1683. (IF: 7.36, # Corresponding authors) (Front Cover).

Highlighted in Science (Ferrarelli LK, 2017, Kiss-and-run or a full commitment? 356(6344):1243-1245. doi: 10.1126/science.356.6344.1243-p).

8.      Zhenli Xie, Jiangang Long, Jiankang Liu, Zuying Chai#, Xinjiang Kang#, Changhe Wang#. (2017) Molecular mechanisms for the coupling of endocytosis to exocytosis in neurons. Frontiers in Molecular Neuroscience. 10:47. doi:10.3389/fnmol.2017.00047. (IF: 5.2, # Corresponding authors) (Invited review)

9.      Zuying Chai, Yang Chen, Changhe Wang# (2017) β-arrestin-1: bridging GPCRs to active TRP channels. Channels. 11(5):357-9. (IF: 2.54, # Corresponding author) (Invited commentary)

10.  Changhe Wang*, Yeshi Wang*, Meiqin Hu*, Zuying Chai, Qihui Wu, Rong Huang, Weiping Han, Claire Xi Zhang, Zhuan Zhou. (2016) Synaptotagmin 11 inhibits clathrin-mediated and bulk endocytosis. EMBO Reports. 17(1):47-63. (IF: 9.06, *Co-first authors) (Front Cover)

11.  Shujiang Shang, Feipeng Zhu, Bin Liu, Zuying Chai, Qihui Wu, Meiqin Hu, Yuan Wang, Rong Huang, Xiaoyu Zhang, Xi Wu, Lei Sun, Yeshi Wang, Li Wang, Huadong Xu, Sasa Teng, Bing Liu, Lianghong Zheng, Chen Zhang, Fukang Zhang, Xinghua Feng, Desheng Zhu, Changhe Wang#, Tao Liu#, Michael Xi Zhu#, Zhuan Zhou#. (2016) Intracellular TRPA1 Mediates Ca2+ Release from lysosomes in dorsal root ganglion neurons. The Journal of Cell Biology. 215(3):369-381. (IF: 8.72, # Corresponding authors)

Highlighted in Journal of Cell Biology (Mingxue Gu and Haoxing Xu, A painful TR(i)P to lysosomes, 2016, 215(3):309-312).

12.  Haiqiang Dou*, Changhe Wang*, Xi Wu, Lijun Yao, Xiaoyu Zhang, Sasa Teng, Huadong Xu, Bin Liu, Qihui Wu, Quanfeng Zhang, Meiqin Hu, Yeshi Wang, Li Wang, Yi Wu, Shujiang Shang, Xinjiang Kang, Lianghong Zheng, Jin Zhang, Matthieu Raoux, Jochen Lang, Qing Li, Jing Su, Xiao Yu, Liangyi Chen, Zhuan Zhou. (2015) Calcium influx activates adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic beta cells. Diabetologia. 58(2):324-33. (IF: 6.67, *Co-first authors) (被该刊编辑部选为‘Up front’特别推荐论文).

13.  Huadong Xu, Panli Zuo, Shirong Wang, Li Zhou, Xiaoxuan Sun, Meiqin Hu, Bin Liu, Qihui Wu, Haiqiang Dou, Bing Liu, Feipeng Zhu, Sasa Teng, Xiaoyu Zhang, Li Wang, Qing Li, Mu Jin, Xinjiang Kang#, Wei Xiong, Changhe Wang#, Zhuan Zhou#. (2015) Striatal dopamine release in a schizophrenia mouse model measured by electrochemical amperometry in vivo. Analyst. 140:3840-3845. (IF: 4.11, # Corresponding authors)

14.  Li Wang, Shujiang Shang, Xinjiang Kang, Sasa Teng, Feipeng Zhu, Bin Liu, Qihui Wu, Mingli Li, Wei Liu, Huadong Xu, Li Zhou, Ruiying Jiao, Haiqiang Dou, Panli Zuo, Xiaoyu Zhang, Lianghong Zheng, Shirong Wang, Changhe Wang# and Zhuan Zhou#. (2014) Modulation of dopamine release in the striatum by physiologically relevant level of nicotine. Nature Communications. 5:3925. DOI: 10.1038/ncomms4925. (IF: 11. 47, # Corresponding authors)

15.  Shujiang Shang*, Changhe Wang*, Bin Liu, Qihui Wu, Quanfeng Zhang, Wei Liu, Lianghong Zheng, Huadong Xu, Xinjiang Kang, Xiaoyu Zhang, Yeshi Wang, Hui Zheng, Shirong Wang, Wei Xiong, Tao Liu, Zhuan Zhou. (2014) Extracellular Ca2+ per se inhibits quantal size of catecholamine release in adrenal slice chromaffin cells. Cell Calcium. 56(3):202-7. (IF: 4.3, *Co-first authors)

16.  Mingli Li, Huadong Xu, Guoqing Chen, Suhua Sun, Qinglong Wang, Bing Liu, Xi Wu, Li Zhou, Zuying Chai, Xiaoxuan Sun, Yang Lu, Muhammad Younus, Lianghong Zheng, Feipeng Zhu, Hongbo Jia, Xiaowei Chen, Changhe Wang and Zhuan Zhou. (2019) Impaired D2 receptor-dependent dopaminergic transmission in prefrontal cortex of awake mouse model of Parkinson’s disease. Brain. doi: 10.1093/brain/awz243. (Epub ahead of print, IF: 11.81).

17.  Quanfeng Zhang, Bin Liu, Qihui Wu, Bing Liu, Yinglin Li, Suhua Sun, Yuan Wang, Xi Wu, Zuying Chai, Xiaohan Jiang, Xiaoyao Liu, Meiqin Hu, Yeshi Wang, Yunting Yang, Li Wang, Xinjiang Kang,1 Yingfei Xiong, Yang Zhou, Xiaoke Chen, Lianghong Zheng, Bo Zhang, Changhe Wang, Feipeng Zhu, and Zhuan Zhou. (2019) Differential co-release of two neurotransmitters from a vesicle fusion pore in mammalian adrenal chromaffin cells. Neuron. 102(1):173-83. (IF: 14.32).

18.  Qihui Wu, Quanfeng Zhang, Bin Liu, Yinglin Li, Xi Wu, Shuting Kuo, Lianghong Zheng, Changhe Wang, Feipeng Zhu and Zhuan Zhou. (2019) Dynamin-1 restrains vesicular release to a sub-quantal mode in mammalian adrenal chromaffin cells. J Neurosci. 39(2):199-211. (IF: 5.97).

19.  Zhuan Zhou, Zuying Chai, Changhe Wang. (2018) From structure to function: unveiling the structure of the Nav channel–toxin complex. Natl Sci Rev. doi: 10.1093/nsr/nwy090 (Epub ahead of print, IF: 13.22).

20.  Yingfei Xiong, Sasa Teng, Lianghong Zheng, Suhua Sun, Jie Li, Ning Guo, Mingli Li, Li Wang, Feipeng Zhu, Changhe Wang, Zhiren Rao, Zhuan Zhou. (2018) Stretch-induced Ca2+ independent ATP release in hippocampal astrocytes. J Physiol. 596: 1931-47. (IF: 4.74)

21.  Chen Hou, Yongyao Wang, Jiankang Liu, Changhe Wang, Jiangang Long. (2017) Neurodegenerative disease related proteins have negative effects on SNARE-mediated membrane fusion in pathological confirmation. Front Mol Neurosci. 10:66. DOI: 10.3389/fnmol.2017.00066. (IF: 5.2)

22.  Linling He, Quanfeng Zhang, Liecheng Wang, Jingxia Dai, Changhe Wang, Lianghong Zheng, Zhuan Zhou. (2015) Muscarinic inhibition of nicotinic transmission in neurons and adrenal chromaffin cells. Philos Trans R Soc Lond B Biol Sci. 370:20140188. DOI: 10.1098/rstb.2014.0188. (IF: 7.06)

23.  Xinjinag Kang, Huadong Xu, Sasa Teng, Xiaoyu Zhang, Zijun Deng, Li Zhou, Panli Zuo, Bing Liu, Bin Liu, Qihui Wu, Li Wang, Meiqin Hu, Haiqiang Dou, Wei Liu, Feipeng Zhu, Qing Li, Shu Guo, Qian Lei, Jing Lv, Yu Mu, Mu Jin, Shirong Wang, Wei Jiang, Kun Liu, Changhe Wang, Weilin Li, Kang Zhang, Zhuan Zhou. (2014) Dopamine release from transplanted neural stem cells in Parkinsonian rat striatum in vivo. Proc Natl Acad Sci USA. 111(44):15804-9. (IF: 9.81) (Weekly press tipsheet特别推荐论文)

24.  Li Wang, Xiaoyu Zhang, Huadong Xu, Li Zhou, Ruiying Jiao, Wei Liu, Feipeng Zhu, Xinjiang Kang, Bin Liu, Sasa Teng, Qihui Wu, Mingli Li, Haiqiang Dou, Panli Zuo, Changhe Wang, Shirong Wang and Zhuan Zhou. (2014) Temporal components of cholinergic terminal to dopaminergic terminal transmission in dorsal striatum slices of mice. J Physiol. 592:3559-76. (IF: 5.04)

25.  Lina Liu, Feipeng Zhu, Meiying Song, Xinjiang Kang, Shujiang Shang, Xiaoyu Zhang, Huadong Xu, Sasa Teng, Bin Liu, Shuting Kuo, Wei Liu, Mingli Li, Li Zhou, Ruiying Jiao, Changhe Wang, Shirong Wang, Hui Yang, Bo Zhang, Zhuan Zhou, Zhiqing Xu. (2014) Effect of Cocaine on Ion Channels and Glutamatergic EPSCs in Noradrenergic Locus Coeruleus Neurons. J Mol Neurosci. 53(3):345-51. (IF: 2.76)

26.  Shuting Kuo, Qihui Wu, Bin Liu, Zhenli Xie, Xi Wu, Shujiang Shang, Xiaoyu Zhang, Xinjiang Kang, Lina Liu, Feipeng Zhu, Yeshi Wang, Meiqin Hu, Huadong Xu, Li Zhou, Bin Liu, Zuying Chai, Quanfeng Zhang, Wei Liu, Sasa Teng, Changhe Wang, Ning Guo, Haiqiang Dou, Panli Zuo, Lianghong Zheng, Claire Xi Zhang, Desheng Zhu, Li Wang, Shirong Wang, Zhuan Zhou. (2014) Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese university. J Mol Neurosci. 53(3):487-92. (IF: 2.76)

27.  Tao Liu, Shujiang Shang, Bin Liu, Changhe Wang, Yeshi Wang, Wei Xiong, Lianghong Zheng, Claire Xi Zhang, Zhuan Zhou. (2011) Two distinct vesicle pools for depolarization-induced exocytosis in somata of dorsal root ganglion neurons. J Physiol. 589 (14): 3507-15. (IF: 4.72)

 

28.  Tao Liu, Lei Sun, Yingfei Xiong, Shujiang Shang, Ning Guo, Sasa Teng, Yeshi Wang, Bin Liu, Changhe Wang, Li Wang, Lianghong Zheng, Claire Xi Zhang, Weiping Han, Zhuan Zhou. (2011) Calcium triggers exocytosis from two types of organelles in a single astrocyte. J Neurosci. 31(29): 10593-601. (IF: 7.12)